Coding

Part:BBa_K2603003:Design

Designed by: Evandro José Mulinari   Group: iGEM18_USP-EEL-Brazil   (2018-10-09)


Lac TT - Laccase from Thielavia terrestris with TEV site, Trx and HisTag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 557
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 557
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1258


Design Notes

This part was desinged to contained a TEV cleavage site and HisTag so that purification can be easily carried out.


Source

Laccase gene sequence from Thielavia terrestris NRRL 8126 was taken from NCBI.

References